Venous blood samples were collected from all participants on the day they were invited to participate in this study to determine the levels of HbA1c, TSH, FT3, FTcuatro, and antithyroid antibodies and the lipid profile. The hemoglobin A1c (HbA1c) level was measured using the National Glycohemoglobin Standardization Program (NGSP)-standardized and Diabetes Control and Complications Trial (DCCT)-compliant immune turbidimetric assay (Hoffman-La Roche Ltd., Basel, Switzerland), with an inter-measurement coefficient of variation of 1.64% according to the manufacturer’s specifications. The reference range was 4.8–6.4% . Lipid profile was assessed using spectrophotometry (enzymatic-colorimetric), and the following reference values were set: total cholesterol 50 mg/dL.
Antithyroid peroxidase (anti-TPO) and antithyroglobulin (anti-Tg) antibodies were detected in venous blood samples using the electrochemiluminescence immunoassay (ECLIA). The upper normal limit for the anti-TPO antibody was set at 34 UI/mL and for the anti-Tg antibody at 115 U/mL . Values greater than these cut-off values indicated positive results. For the evaluation of thyroid function, TSH, FT4, and FT3 levels were measured using ECLIA. The following reference values were used: TSH = 0.465–4.68 Miu/mL, FT3 = 3.69–10.4 pmol/L, and FT4 = 10–28.2 pmol/L . Thyroid ultrasonography was performed in all cases to measure the thyroid volume and study the changes in parenchymal density. The presence of an enlarged thyroid (thyroid gland volume > 97th age-related percentile) with diffuse hypo-echogenicity and/or diffuse micronodules confirmed the diagnosis of autoimmune thyroiditis.
The brand new medical diagnosis away from retinopathy was established once funduscopic test used from the a trained ophthalmologist just who specialized in diabetic eye difficulty. Podiatric test is performed depending on the standardized protocol and integrated assessments out of vibrations and you can tactile, serious pain, and you will thermic feelings; photoplethysmography; and you will dimension of ankle and you can bottom hypertension to determine the ankle/brachial and you can bottom/brachial indexes, respectively. Ewing’s electric battery sample are performed to confirm this new diagnosis off cardiac autonomic neuropathy. Oftentimes, echocardiography and you can Doppler ultrasound of your carotid artery were used to help you influence the severity of macrovascular problem.
Evaluation regarding sexual dysfunction
The first FSDS level was designed to scale distress, that’s probably one of the most very important apparent symptoms of FSD . We made use of the modified adaptation (FSDS-R), comprising thirteen issues, where level of distress is measured since a regularity. Brand new response level for every question’s goods ranges anywhere between 0 (never) and you can cuatro (always). What exactly try summed up to locate a whole rating varying off 0 so you’re able to 52. The fresh new slashed-regarding rating are ? 11 .
The newest European type of new FSFI is translated, reviewed, and you may approved by the Lookup Panel of your own Timisoara State Disaster Healthcare for usage into Romanian inhabitants. The brand new FSFI includes 19 inquiries into six top features of the new lady intimate response: attention, stimulation, oiling, orgasm, pleasure, and you will pain. The new answers are examined based on how often for every single ability happen. Towards the FSFI, the minimum rating is 2 as well as the restrict try thirty six, to your slash-regarding rating out of twenty six.5 . The size and style has concerns into the six domains, specifically interest, subjective stimulation, oiling, climax, satisfaction, and you can problems. Anyone domain name scores are listed below: step one.2–six to possess focus; 0–six to have arousal, oiling, climax, and you can pain; and you may 0.8–6 to have fulfillment. Reduced ratings indicate higher danger signal weight . The fresh new patients have been asked to complete this type of questionnaires in the home and you may go back her or him once 30 days. People whom did not return new questionnaire timely was delivered a note via cell phone.
Evaluation of depression
To possess assessing the degree of anxiety, we used the BDI-II scale , comprising 21 inquiries, which have the absolute minimum score off 0 and you will a total of 63, and you may a cut right out-out-of rating off ? 17 . Brand new clients have been asked to do which questionnaire at your home and you can send it back once 4 weeks. People which don’t come back the latest questionnaire on time was basically sent an indication through cellular phone.